XWH - 11 - 1 - 0607 TITLE : iPSC - Derived MSCs that Are Genetically Engineered for Systemic Bone
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Award Number : W 81 XWH - 11 - 1 - 0607 TITLE : iPSC - Derived MSCs that Are Genetically Engineered for Systemic Bone
The ability to efficiently generate integration-free induced pluripotent stem cells (iPSCs) from the most readily availablesource—peripheral blood—has the potential to expedite the advances of iPSC-based therapies. We have successfullygenerated integration-free iPSCs from cord blood (CB) CD34 cells with improved oriP/EBNA1-based episomal vectors (EV)using a strong spleen focus formi...
متن کاملReprogramming of mesenchymal stem cells derived from iPSCs seeded on biofunctionalized calcium phosphate scaffold for bone engineering.
Human induced pluripotent stem cell-derived mesenchymal stem cells (iPSC-MSCs) are a promising choice of patient-specific stem cells with superior capability of cell expansion. There has been no report on bone morphogenic protein 2 (BMP2) gene modification of iPSC-MSCs for bone tissue engineering. The objectives of this study were to: (1) genetically modify iPSC-MSCs for BMP2 delivery; and (2) ...
متن کاملTherapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study
BACKGROUND We derived mesenchymal stem cells (MSCs) from rat induced pluripotent stem cells (iPSCs) and transduced them with tumor necrosis factor alpha-stimulated gene-6 (TSG-6), to test whether TSG-6 overexpression would boost the therapeutic effects of iPSC-derived MSCs in experimental periodontitis. METHODS A total of 30 female Sprague-Dawley (SD) rats were randomly divided into four grou...
متن کاملGenetically Engineered Mesenchymal Stem Cells Stably Expressing Green Fluorescent Protein
Objective(s) Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this stu...
متن کاملiPSC‐derived mesenchymal stem cells exert SCF‐dependent recovery of cigarette smoke‐induced apoptosis/proliferation imbalance in airway cells
Mesenchymal stem cells (MSCs) have emerged as a potential cell-based therapy for pulmonary emphysema in animal models. Our previous study demonstrated that human induced pluripotent stem cell-derived MSCs (iPSC-MSCs) were superior over bone marrow-derived MSCs (BM-MSCs) in attenuating cigarette smoke (CS)-induced airspace enlargement possibly through mitochondrial transfer. This study further i...
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تاریخ انتشار 2012